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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha : |
07/06/2019 |
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Actualizado : |
18/11/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
IRISARRI, P.; CARDOZO, G.; TARTAGLIA, C.; REYNO, R.; GUTIÉRREZ, P.; LATTANZI, F.; REBUFFO, M.; MONZA, J. |
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Afiliación : |
PILAR IRISARRI, Laboratorio de Microbiología, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY; GERONIMO AGUSTIN CARDOZO CABANELAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA TARTAGLIA, Laboratorio de Bioquimica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, Uruguay.; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PAMELA GUTIÉRREZ, Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY.; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE MONZA, Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, UDELAR, URUGUAY. |
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Título : |
Selection of competitive and efficient rhizobia strains for white clover. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Frontiers in Microbiology, 23 April 2019, v. 10, art. 768. DOI: https://doi.org/10.3389/fmicb.2019.00768 |
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Páginas : |
11 p. |
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DOI : |
10.3389/fmicb.2019.00768 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 September 2018 // Accepted 26 March 2019 // Published: 23 April 2019. Open Access Journal. Acknowledgments: We are grateful to Verónica Berriel for the N abundance determination and to Carlos Rossi for providing white clover seeds. |
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Contenido : |
The practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupancy of the native-naturalized experimental strains was at least five times greater. The amount of N fixed per unit plant mass was consistently higher with native-naturalized experimental strains, although the proportion of N derived from atmospheric fixation was similar for all strains. Plant establishment and herbage production, as well as clover contribution in oversown native grasslands, were either similar or higher in white clover inoculated with the native-naturalized experimental strains. These results support the use of our implemented strategy for developing a competitive inoculant from native-naturalized strains. MenosThe practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupan... Presentar Todo |
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Palabras claves : |
BIOLOGICAL NITROGEN FIXATION; INOCULANT; NATIVE-NATURALIZED RHIZOBIA STRAINS; NODULE OCCUPANCY; R. LEGUMINOSARUM SV. TRIFOLII; WHITE CLOVER. |
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Thesagro : |
FIJACION BIOLOGICA DEL NITRÓGENO; NODULACION; TREBOL BLANCO; TRIFOLIUM REPENS. |
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Asunto categoría : |
F60 Fisiología y bioquímica de la planta |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12797/1/Cardozo-Frontier-Micro-2019.pdf
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Marc : |
LEADER 03468naa a2200361 a 4500
001 1059873
005 2021-11-18
008 2019 bl uuuu u00u1 u #d
024 7 $a10.3389/fmicb.2019.00768$2DOI
100 1 $aIRISARRI, P.
245 $aSelection of competitive and efficient rhizobia strains for white clover.$h[electronic resource]
260 $c2019
300 $a11 p.
500 $aArticle history: Received 28 September 2018 // Accepted 26 March 2019 // Published: 23 April 2019. Open Access Journal. Acknowledgments: We are grateful to Verónica Berriel for the N abundance determination and to Carlos Rossi for providing white clover seeds.
520 $aThe practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In consequence, failures in establishment, and low productivity attributed to poor performance of the inoculant may merely reflect the absence of the inoculated strain in the nodules. This study lays out a strategy followed for selecting a Rhizobium leguminosarum sv. trifolii strain for white clover (Trifolium repens) with competitive nodule occupancy. First, the competitiveness of native-naturalized rhizobia strains selected for their efficiency to fix N2 in clover and tagged with gusA was evaluated in controlled conditions with different soils. Second, three of these experimental strains with superior nodule occupancy plus the currently recommended commercial inoculant, an introduced strain, were tested in the field in 2 years and at two sites. Plant establishment, herbage productivity, fixation of atmospheric N2 (15N natural abundance), and nodule occupancy (ERIC-PCR genomic fingerprinting) were measured. In both years and sites, nodule occupancy of the nativenaturalized experimental strains was either higher or similar to that of the commercial inoculant in both primary and secondary roots. The difference was even greater in stolon roots nodules, where nodule occupancy of the native-naturalized experimental strains was at least five times greater. The amount of N fixed per unit plant mass was consistently higher with native-naturalized experimental strains, although the proportion of N derived from atmospheric fixation was similar for all strains. Plant establishment and herbage production, as well as clover contribution in oversown native grasslands, were either similar or higher in white clover inoculated with the native-naturalized experimental strains. These results support the use of our implemented strategy for developing a competitive inoculant from native-naturalized strains.
650 $aFIJACION BIOLOGICA DEL NITRÓGENO
650 $aNODULACION
650 $aTREBOL BLANCO
650 $aTRIFOLIUM REPENS
653 $aBIOLOGICAL NITROGEN FIXATION
653 $aINOCULANT
653 $aNATIVE-NATURALIZED RHIZOBIA STRAINS
653 $aNODULE OCCUPANCY
653 $aR. LEGUMINOSARUM SV. TRIFOLII
653 $aWHITE CLOVER
700 1 $aCARDOZO, G.
700 1 $aTARTAGLIA, C.
700 1 $aREYNO, R.
700 1 $aGUTIÉRREZ, P.
700 1 $aLATTANZI, F.
700 1 $aREBUFFO, M.
700 1 $aMONZA, J.
773 $tFrontiers in Microbiology, 23 April 2019$gv. 10, art. 768. DOI: https://doi.org/10.3389/fmicb.2019.00768
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Registro original : |
INIA Tacuarembó (TBO) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Treinta y Tres. |
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Fecha actual : |
26/09/2014 |
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Actualizado : |
14/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 1 |
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Autor : |
ABREO, E.; MARTINEZ, S.; SESSA, L.; BETTUCCI, L.; LUPO, S. |
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Afiliación : |
SEBASTIAN MARTINEZ KOPP, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Phomopsis cotoneastri as a pathogen associated with trunk cankers and death of young apple tree cv. Cripps Pink. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Journal of Phytopathology. 2012. v.160, no.7-8, p. 434-436. |
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ISSN : |
0931-1785 |
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DOI : |
10.1111/j.1439-0434.2012.01914.x |
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Idioma : |
Inglés |
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Notas : |
Article history: Received December 21, 2011; accepted March 31, 2012.
https://doi.org/10.1111/j.1439-0434.2012.01914.x |
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Contenido : |
ABSTRACT.
This is the first report of Phomopsis cotoneastri as a pathogen isolated from sunken cankers in trunks of young apple trees cv. Cripps Pink that had died in the first year after planting. Phomopsis cotoneastri was identified by sequence analysis of part of the translation elongation factor 1-alpha gene (EF1-α). Pathogenicity of two isolates of P. cotoneastri was demonstrated in field conditions.
© 2012 Blackwell Verlag GmbH. |
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Thesagro : |
FACTOR DE ELONGACION; MANZANO; PHOMOPSIS COTONEASTRI. |
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Asunto categoría : |
--
A50 Investigación agraria |
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Marc : |
LEADER 01284naa a2200241 a 4500
001 1050607
005 2019-10-14
008 2012 bl uuuu u00u1 u #d
022 $a0931-1785
024 7 $a10.1111/j.1439-0434.2012.01914.x$2DOI
100 1 $aABREO, E.
245 $aPhomopsis cotoneastri as a pathogen associated with trunk cankers and death of young apple tree cv. Cripps Pink.$h[electronic resource]
260 $c2012
500 $aArticle history: Received December 21, 2011; accepted March 31, 2012. https://doi.org/10.1111/j.1439-0434.2012.01914.x
520 $aABSTRACT. This is the first report of Phomopsis cotoneastri as a pathogen isolated from sunken cankers in trunks of young apple trees cv. Cripps Pink that had died in the first year after planting. Phomopsis cotoneastri was identified by sequence analysis of part of the translation elongation factor 1-alpha gene (EF1-α). Pathogenicity of two isolates of P. cotoneastri was demonstrated in field conditions. © 2012 Blackwell Verlag GmbH.
650 $aFACTOR DE ELONGACION
650 $aMANZANO
650 $aPHOMOPSIS COTONEASTRI
700 1 $aMARTINEZ, S.
700 1 $aSESSA, L.
700 1 $aBETTUCCI, L.
700 1 $aLUPO, S.
773 $tJournal of Phytopathology. 2012.$gv.160, no.7-8, p. 434-436.
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INIA Las Brujas (LB) |
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